Bed Height Chromatography

The 200 μL column volume is recommended for sorbent and process conditions screening while the 600 μL is used for purification process optimization or when a.

Bed height chromatography. CF is used in the medium preparation step to calculate the medium volume needed to pack a desired bed height. Doubling the bed height gives an increase in resolution equivalent to 2 14 40. Two formats are available 200 μL and 600 μL with a bed height of 10 and 30 mm respectively.

Column efficiency is related to the band broadening that can occur on a column and can be calculated from the expression. Recommended Choose your own. Adjustable bed height Bed volume 15 ml 15 ml 012307 ml 19219 ml Bed height 25 cm 210 cm 0247 cm 5124 cm Maximum operating pressure 72 psi05 MPa 290435 psi23 MPa 150900 psi 103620 MPa 5001000 psi.

Sartobind Q strong ion exchangers are perfect tools for the polishing of monoclonal antibodies for the removal of viruses DNA host cell proteins leached Protein A and endotoxins. 3 See Figure 1 and section 7 Cleaning-In-Place CIP. Chromatography volume flow rate calculator Contact.

Column efficiency is expressed as the number of theoretical plates per meter chromatography bed N or as H height equivalent to a theoretical plate HETP which is the bed length L divided by the plate number. Then what is bed volume. However at large scale chromatography systems can generally deliver flow rates corresponding to linear velocities 400 cmh for 1 m diameter columns or larger.

Packed bed dimensions. Packed bed chromatography columns are usually used to purify drug products. Residence time is 24 min.

For high resolution and fractionation long columns will give the best results. ScreenExpert RoboColumns are packed with anion exchange mixed-modeaffinity or any other chromatography sorbents upon request. Packed bed height cm Column volume V C L packed A C where A C cross-sectional area of the column cm2 When packing BPG and AxiChrom columns PF is used in the packing procedure to calculate the packed bed height after the consolidation step.